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Tb green® premix ex taqtm ii kit

WebJul 22, 2024 · RNA was extracted from equal volume of the five combined fractions using TRIzol reagents (Life Technologies, Shanghai, China), reversely transcribed to cDNA using the PrimeScriptTM RT reagent Kit (Takara, Beijing, China) and amplified by qTR-PCR analysis using the TB Green Premix Ex TaqTM II kit (Takara, Beijing, China). WebOct 7, 2024 · The target gene and β-actin expression levels were detected using the TB Green ® Premix Ex TaqTM II Kit (TaKaRa, Tokyo, Japan). The reaction was performed …

TB Green Premix Ex Taq II (Tli RNase H Plus) by Takara Bio

WebSep 1, 2024 · JC-1 Assay Kit, Annexin V-FITC/PI Kit, enhanced ATP Assay Kit, and Bicinchoninic Acid (BCA) Assay Kit were obtained from Beyotime (China). FerroOrange, … WebSep 21, 2024 · The purity of total RNA was evaluated using spectrophotometry. cDNA was then synthesized using a PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa, China). RT-qPCR was performed using TB Green® Premix Ex TaqTM II (TaKaRa, China) according to the manufacturer’s instructions. peerless kitchen faucets repair https://sh-rambotech.com

Premix Ex Taq DNA Polymerase for Real-Time PCR

WebTaKaRa tb green tm premix ex taq tm ii pcr kit Tb Green Tm Premix Ex Taq Tm Ii Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on … WebApr 13, 2024 · An ABI PRISM 7500 instrument and a TB Green ® Premix Ex TaqTM II kit (TaKaRa, Tokyo, Japan) were used to perform qRT-PCR. Using the UBC gene as an internal control [ 38 ]. Primer Premier 3.0 was used to create primers for qRT-PCR reactions; the primer sequences are provided in Table S3 . WebTB Green® Premix Ex Taq™ II (Tli RNase H Plus), 5 x 5 mL, ROX Plus. 1,000 Rxns. USD $1460.00. License Statement. ID Number. M82. This product is covered by the claims of … meat carving knife review

Genome-Wide Identification of Seven Polyamine Oxidase Genes in

Category:TB Green Premix Ex Taq II (Tli RNase H Plus) by Takara Bio

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Tb green® premix ex taqtm ii kit

TB Green Premix Ex Taq (Tli RNase H Plus) - Takara Bio

WebMar 1, 2024 · TB Green ® Premix Ex Taq ™ II (Tli RNaseH Plus) 40 Rxns ￥525: Takara RR820A TB Green ® Premix Ex Taq ™ II (Tli RNaseH Plus) 200 Rxns ￥1,785 ￥893: … WebTakara SYBR Green Master Mix products contain TaKaRa Ex Taq HS DNA Polymerase, a hot start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq antibody allows highly reproducible and reliable qPCR. SYBR Premix Ex Taq II (Tli RNase H Plus) also enables high reaction specificity and superior performance with GC-rich templates.

Tb green® premix ex taqtm ii kit

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WebFeb 3, 2024 · Reverse transcription of total RNA was performed following Prime Script ™ RT reagent Kit with gDNA Eraser (Takara, China), TB Green ® Premix Ex TaqTM II(TAKARA, Beijing, China) instructions. The reverse transcription and qPCR of miRNA were performed with miRcute Plus miRNA First-Strand cDNA Kit and miRNA Plus miRNA qPCR Kit … WebJul 16, 2024 · Using GAPDH as an internal reference gene, RT-qPCR was performed using TB Green TM Premix Ex TaqTM II (Takara). The reaction system was as follows: TB Green Premix Ex Taq II (Tli RNaseH Plus) 10 μL, PCR forward primer (10 μM) 0.8 μL, PCR reverse primer (10 μM) 0.8 μL, DNA (< 100 ng) 2 μL, and sterilized water 6.4 μL.

WebTakara SYBR Green Master Mix products contain TaKaRa Ex Taq HS DNA Polymerase, a hot start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq …

WebApr 11, 2024 · The cDNAs were generated using GoScript™ Reverse Transcription Kit (Promega, Madison, WI, USA). The mRNA levels of DEPTOR were tested using TB Green Premix Ex TaqTM II (Takara Bio) on the QuantStudio 5 real-time PCR system (Applied Biosystems, Foster City, CA, USA). GAPDH was used for data normalization. WebDec 21, 2024 · RNA of L. cubeba was extracted and reverse transcribed by the method provided by Zhao et al. (2024). qRT-PCR was carried out with the assistance of an ABI PRISM 7500 instrument and the TB Green ® Premix Ex TaqTM II kit.

WebAfter denatured template DNA at 95°C for 10 s, 40 cycles of 95°C for 5 s, and 60°C for 20 s were performed using TB Green ® Premix Ex TaqTM II Kit (TAKARA), 21 on the ABI StepOne/StepOnePlus Real-Time System.

WebPrimeScriptTM RT reagent Kit with gDNA Eraser (RR047A, Takara, Shiga, Japan) was used for reverse transcription. TB Green® Premix Ex TaqTM II (RR820A, Takara, Shiga, Japan) was used for quantitative PCR in the QuantStudioTM 3 Real-Time PCR System (Thermo Fisher, Carlsbad, CA). Primers were obtained from Thermo Fisher. peerless kitchen faucet sprayer replacementWebSep 4, 2024 · TaKaRa TB Green ® Premix Ex TaqTM II kit (TAKARA Bio, Japan) was chosen for qRT-PCR, and the instrument used was QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Singapore). Every sample was tested in three biological replicates and three technical replicates, which showed good consistency. meat carving knife setsWeb36673306 bids are invited for takara chemicals mse emeraldamp gt pcr master mix , primescript tm first strand cdna synthesis kit , agarose routine 00g , tb green premix ex taqtm ii tli rnaseh plus , one kb dna ladder dye plus total quantity : 5 peerless ladysmith hours and days openWebMay 21, 2024 · Total RNAs were isolated from cells using the TRIzol reagent and reverse-transcribed into cDNAs using PrimeScript TM RT reagent Kit. Quantitative PCR (qPCR) … peerless kitchen three handle leakWebPremix Ex Taq (Probe qPCR) is a 2X master mix for real-time PCR (qPCR) using probe-based qPCR or 5' nuclease assays. This 2X master mix includes Takara Ex Taq HS—a … meat carving knife setWebDec 11, 2013 · Premix Ex Taq是一种2浓度的Premix Type试剂，进行实验时，PCR反应液的配制十分方便简单。. Mix 中添加了Tli RNaseH（耐热性RNaseH），以cDNA作为模板 … peerless kitchen sprayer hose removal youtubeWebFeb 1, 2024 · TB Green® Premix Ex TaqTM II (Tli RNase H Plus) was used for real-time PCR analysis performed on Applied Biosystems (Thermo Fisher Scientific, Waltham, MA, USA). The following primers were used: F 5′-TCCTTCAGACACCCTCAAC-3′, R 5′-CAGGGATCAAAGCTGTAGGC-3′ for TNF-α , F 5′-GAAATCCCATCACCATCTTCCAGG … peerless kitchens duluth ga