WebRNA Circularization using T4 RNA Ligase 1 Protocol for ssDNA/RNA Ligation (M0319) Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1 3´ End Labeling of RNA using T4 RNA Ligase 1 Typical Protocol for RNA Ligation (M0458) Featured Products T4 RNA Ligase 2, truncated K227Q T4 RNA Ligase 1 (ssRNA Ligase) WebB6030 (10X T4 DNA Ligase Buffer) 10X T4 DNA Ligase Buffer (B6030) 500mM Tris-HCI 100 mM MgCl 2 50 mM DTT 10 mM ATP pH 7.6 @ 25°C Unit Definition 1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X T4 DNA Ligase Buffer following a 30 minute incubation at …
Can T4 DNA Ligase be heat inactivated? NEB
WebT4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky … WebT4 RNA Ligase 1 Add to Cart: L6050L: 10,000 U: 20,000 U/ml: $443: Single-stranded RNA ligase. Also joins single-stranded DNA molecules. Add to Cart: T4 DNA Ligase (Rapid) Add to Cart: L6030-HC-L: 240,000 U: 600,000 U/ml: $707 “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008). Add to Cart: T3 DNA Ligase Add to Cart ... pei green party leader
Hi all, for a ligation reaction, is it true that incubating the ...
WebT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. WebGlycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50U/µL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate and 3' hydroxyl groups in duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. WebThe T4 DNA Ligase requires ATP as a cofactor. Highlights. Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) Fast – sticky … pei great white shark