WebMar 18, 2024 · Using FaST-PLIM, we image the cellular motility of T-lymphocytes in relation to the microscopic distribution of oxygen in mouse models of hematological and solid tumors, namely in bone marrow with or without B-cell acute lymphocytic leukemia (ALL), and in lungs with sarcoma tumors. WebFeb 15, 2024 · When the temperature increased from 22 to 28 °C, the phosphorescence lifetime of 3 at 482 nm extends from 321 to 448 ns. When a time gate of 150 ns was used …
Wide-field time-correlated single photon counting-based …
WebOperation of the microscope is through a microprocessor interfaced keypad by which all timing parameters can be adjusted for optimal suppression of fast decaying luminescence. Accuracy of the timing was within 1 μs. Prompt fluorescence was suppressed up to 10 6 times, as determined for bright prompt fluorescing microspheres. The use of the FLC ... WebJan 9, 2024 · The phosphorescence lifetime of Pt [L]Cl is sensitive to viscosity and provides an excellent tool to investigate the impact of DNA damage. We show using Fluorescence Lifetime Imaging (FLIM)... chris jensen attorney
Phosphorescence Lifetime Imaging Microscopy (PLIM) …
WebDec 30, 2024 · FLIM is available in two primary modes of operation, time-domain [5] and frequency domain [6], but also is compatible with a number of different microscopy … WebSep 6, 2024 · The data shows one of the first examples of non-invasive, real-time oxygen mapping across a melanoma tumour spheroid using one-photon phosphorescence lifetime imaging microscopy (PLIM) and a small ... WebJun 1, 2024 · For phosphorescence imaging or phosphorescence lifetime measurements in HK-2 cells, all cells were cultured on glass-base dishes. After 2-hour incubation with BTPDM1, the media was changed to those containing antimycin A (10 μM), followed by PLIM. PLIM and fluorescence lifetime imaging microscopy configurations chris jensen from match game california