Oxidized ldl uptake assay kit
WebHUFI00713. Size: 96 Assays. Alias: OxLDL, Oxidized low-density lipoprotein. Detection method: Sandwich ELISA, Double Antibody. Application: This immunoassay kit allows for the in vitro quantitative determination of Human OxLDL concentrations in serum plasma and other biological fluids. WebApr 16, 2024 · The cholesterol uptake cell-based assay kit and oxidized LDL uptake assay kit were purchased from Cayman. Note that 5 × 10 5 /mL LPS-primed macrophages were incubated in serum-free medium containing 20 μg/mL NBD cholesterol or 1:50 diluted oxLDL-DyLight TM 488 for 2 h at 37 o C. Cells were then trypsinized with 0.25% Trypsin …
Oxidized ldl uptake assay kit
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WebGiven that most NHLs are of B-cell origin, the current study aimed to highlight the association of baseline levels of conjugated dienes in low-density lipoprotein (LDL-BCD) and NO with aggressive B-cell NHL risk and to assess their link with T helper (Th)1/Th2 ratio. In this context, a cross-sectional case–control study was conducted at the ... WebBCA蛋白浓度测定试剂盒 (增强型) (Enhanced BCA Protein Assay Kit)是根据目前世界上最常用的两 种蛋白浓度检测方法之一BCA法研制而成，实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。. 和碧云天生产的普通BCA蛋白浓度测定试剂盒相比，灵敏度更高，检 …
WebAssay kit format. Sandwich ELISA Kit. Detector antibody conjugate. Biotin. Label or dye. HRP . Gene aliases. CLEC8A, LOX1, LOXIN, OLR1, SCARE1, SLOX1. Gene ID (Human) 4973. Gene symbol. ... LOX-1 (lectin-like oxidized LDL receptor -1) is a 40-50 kDa type II membrane glycoprotein class E scavenger receptor expressed by endothelial cells ... WebIn atherosclerotic lesions, MDM are the primary source of NADPH oxidase-derived superoxide anion (O2-) inducing low-density lipoprotein (LDL) oxidation leading to their unregulated uptake of oxidized LDL and foam cell formation. We recently discovered that zymosan potently activates monocyte NADPH oxidase via the non-toll pattern recognition ...
WebCayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The kit employs human LDL conjugated to DyLight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. Web2.1. Purification and Oxidation of LDL. μm), and stored at 4°C. The protein concentration was determined by the BCA kit (Pierce, USA). Oxidized LDL (oxLDL) was obtained by incubation of LDL (2 mg/mL) with 20 μM CuSO 4 for 18 h at 37°C. The oxidation of both protocols was terminated by the addition of 0.5 mM EDTA. 2.2. Human THP-1 Monocytic ...
WebJan 11, 2016 · Background Lectin-like oxidized low-density lipoprotein-1 (LOX-1) is the major receptor for oxidized low density lipoprotein (ox-LDL) uptake in human umbilical vein endothelial cells (HUVECs). Previously, we found that rapamycin inhibited ox-LDL accumulation in HUVECs, and this effect was related to its role in increasing the activity of …
WebDetect oxidized LDL uptake by cultured cells by flow cytometry or fluorescence microscopy. Flow-based readout allows possibility of multiplexing. Inhibitor of oxLDL uptake is … Cayman’s Oxidized LDL Uptake Assay Kit is a convenient tool to study modulators of … Register for Your Account . Manage your data, track shipments, review your order … We collect cookies for vital website function and to better serve our customers. By … geoffrey murphy university of michiganWebMay 6, 2024 · Elevated lipoprotein(a) (Lp[a]) is the single most common genetically inherited risk factor for cardiovascular disease and calcified aortic valve stenosis ().Elevated Lp(a) is common; approximately 25% of the general population has Lp(a) levels in the atherogenic range (i.e., above 30 to 50 mg/dl or 75 to 125 nmol/l) ().Lp(a) is a low-density lipoprotein … geoffrey murryWeb7. STA-389: Human Oxidized LDL ELISA Kit (HNE-LDL Quantitation) ... 9. STA-391: HDL and LDL/VLDL Cholesterol Assay Kit Kit Components 1. Dextran Solution (Part No. 260801): One 8 mL bottle 2. ... Protein deglycosylation can drastically effect the cellular uptake. Nanoscale. doi: 10.1039/C8NR08305C. 7. Frank, A.C. et al. (2024). Apoptotic tumor ... geoffrey murphyWebCompare Oxidized LDL Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. chris mcdonough retired deceiveWebApr 12, 2024 · A glucose uptake assay was conducted in insulin resistance HepG2 (IR-HepG2) cells to establish the antidiabetic activity of the Lc-EAF. ... for biochemical analysis. The levels of glucose, triglycerides, total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT ... geoffrey murray ucdavisWebFoam cell formation as a result of imbalance of modified cholesterol influx and efflux by macrophages is a key to the occurrence and development of atherosclerosis. Oxidative stress is thought to be involved in the pathogenesis of atherosclerosis. SS-31 is a member of the Szeto-Schiller (SS) peptides shown to specifically target the inner mitochondrial … geoffrey munn wartskiWebCayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The kit employs human LDL conjugated to … chris mcdonnell actor