WebMy project focused on studying the development of allo-parental behavior in mice. I used a combination of techniques, including serial two-photon tomography, deep-learning-based cell detection, brain-wide voxel intensity analysis, and channelrhodopsin-2-assisted circuit mapping, to examine the anatomical and functional changes in the hypothalamic … WebSep 29, 2024 · Next, we performed channelrhodopsin (ChR2)-assisted circuit mapping (CRACM) to test synaptic connectivity of these projections to PVH AVP and SON AVP neurons. AAV-DIO-ChR2-mCherry was injected into the SFO or MnPO/OVLT of AVP-GFP;Vglut2-IRES-Cre or AVP-GFP;Vgat-IRES-Cre mice and light-evoked postsynaptic …
A brainstem circuit for nausea suppression - ScienceDirect
WebOct 9, 2024 · Interrogating Neuroendocrine Circuits With ChR2-Assisted Circuit Mapping in Brain Slices. Because ChR2 is trafficked to the axons of targeted neurons, it can be used to define connectivity patterns within neuronal networks . In terms of the neuroendocrine circuits controlling hormone secretion, the ChR2-assisted circuit mapping (CRACM) … WebAug 15, 2016 · To overcome this limitation, Baker et al. developed a new optogenetic approach with two important features. First, the approach makes use of a light-sensitive protein called channelrhodopsin that had … redlining communities
NPGRJ NN 1891 663. - ResearchGate
WebSep 3, 2024 · SUMMARY Channelrhodopsin-assisted circuit mapping (CRACM) is a precision technique for functional mapping of long-range neuronal projections between anatomically and/or genetically identified groups of neurons. Here, we describe how to utilize CRACM to map auditory brainstem connections, including the use of a red-shifted opsin, … WebCRACM, or channelrhodopsin-2 (ChR2)-assisted circuit mapping, is an optical method used to dissect large, complex neural circuits with optogenetics ( Petreanu et al. 2007 ). To … To offer neuroscientists the ability to stimulate and visualize individual … The OASIS Macro is a mesoscope for all-optical targeted optogenetics, calcium … The OASIS Micro is designed to image the activity of thousands of neuronal … Cortical-Circuit Mapping. Learn About the OASIS Macro. Optogenetics and … (In Canada) 111 Railside Road, Suite 201, Toronto, Ontario M3A 1B2, CANADA … Mightex BLS-1000-2, BLS-3000-2, BLS-13000-1, and BLS-18000-1 LED … Mightex BioLED Analog and Digital I/O Control Module is a system-level control … Mightex’s optogenetics starter kits are ready-to-use turn-key solution for ChR2 … (In Canada) 111 Railside Road, Suite 201, Toronto, Ontario M3A 1B2, CANADA … Mightex BLS series BioLED controllers are fully-customizable turn-key solutions for … WebApr 10, 2024 · To confirm a functional connection between cMPOA Esr1 cells and VMHvl Esr1 cells, we performed channelrhodopsin (ChR2)-assisted circuit mapping on brain slices (Fig. 4a). redlining contracts definition