WebFeb 3, 2024 · Cell pellets harvested from T0 and T21 were stored at −80°C for isolation of genomic DNA (gDNA), which was performed with the Blood & Cell Culture DNA Maxi Kit (Qiagen). Two rounds of PCR were performed for identification of sgRNA inserts. WebDec 11, 1998 · The DNA solutions were assayed by absorption measurement at 260 nm and 280 nm and by agarose gel electrophoresis. In a parallel study genomic DNA was isolated from whole blood (200 μl) using the QIAamp Kit (Qiagen) and using the Blood and Cell Culture DNA Mini and Maxi Kits (Qiagen) for 1 ml and 10 ml samples, respectively. …
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WebOct 24, 2011 · Genomic DNA was purified from cells using either the QIAamp DNA Blood Maxi Kit, or the Blood & Cell Culture DNA Maxi Kit (Qiagen Inc., Valencia, CA) according to manufacturer's instructions. Quality was assessed by optical density 260/280 ratio using a spectrophotometer (Beckman-Coulter, Fullerton, CA) and by 0.8% agarose (SeaKem … WebCatalog number: A30706. The JetQuick™ Blood and Cell Culture DNA Maxiprep Kit is designed for rapid and efficient purification of genomic … lc wildcats sports
Genomic DNA Mini Kit (Blood/Cultured Cell) For research …
WebApr 11, 2024 · To simulate an in vitro serum stability test, PP/DNA solution was mixed with BSA, so that the final concentration of BSA in the solution was 10% (commonly used culture conditions for cell culture). The samples were continuously incubated for 7 days, and the same volume of each group was taken out for gel electrophoresis experiments. WebCRISPR Screen: After 28 days, cells were harvested, and genomic DNA was extracted by using the Blood and Cell culture DNA Maxi Kit (QIAGEN) according to manufacturer’s instructions. HiChIP: 15 million cells were cross-linked with 1% formaldehyde for 10 min at room temperature, followed by adding 125mM glycine for 5 min to stop reaction. WebFast Genomic DNA Preparations from Blood Samples The Genomic DNA MINI and MAXI (Blood/Cultured Cell) Kits provide a fast and economical method for the purification of … lc wigs melbourne